Drug Metabolism & Toxicology Studies

Preliminary pharmacokinetic study, early safety evaluation, customized experimental protocols that meet the standards according to the needs of customers, and provide evaluation reports in Chinese and English

Pharmacokinetics

Reveal bioavailability (F), half-life (t1/2), area under the curve (AUC), clearance (CL), peak concentration (Cmax), etc.

In vitro——In development……

Determination of plasma protein binding rate
Plasma stability test
Liver microsome metabolism test
CYP450 inhibition test
CYP450 induction test
Metabolite extrapolation
Metabolite confirmation
Speculation of metabolic pathways
Confirmation of metabolic pathways
Transmembrane transport test
Drug drug interactions
Metabolic phenotype study
Liver cell metabolic stability test

In vivo

Animal species: rat, mouse, etc.
Route of administration: oral, subcutaneous, intramuscular, intravenous
Analysis and detection: LC/GC-MS/MS establishment and optimization
Biological matrix: plasma/serum, tissue, bile, feces, urinary excretion, etc.

Safety evaluation

In vitro

Cytotoxicity studies: MTT colorimetry

Type	Classification	Cell Line
tumor	lung cancer	H522、A549
	breast cancer	MX-1、MCF-7
	stomach cancer	HGC-27
	Pancreatic cancer	BxPC-3
	kidney cancer	A498
	liver cancer	Huh-7、HepG-2
	prostate cancer	LNCap
	ovarian cancer	SK-OV-3
	cervical cancer	Hela

In vivo

Animal species: rat, mouse, etc.
Single-dose toxicity test: maximum tolerated dose (MTD), minimum tolerated dose (MLD), half-lethal dose (LD50), etc.
Multiple-dose toxicity test: 7/14/28-day target organ study, no obvious damaging effect dose (NOAEL), etc.

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